Sexual Reproduction in Flowering Plants Revision Notes

Class 12 Notes

Please refer to the Sexual Reproduction in Flowering Plants Revision Notes given below. These revision notes have been designed as per the latest NCERT, CBSE and KVS books issued for the current academic year. Students will be able to understand the entire chapter in your class 12th Biology book. We have provided chapter wise Notes for Class 12 Biology as per the latest examination pattern.

Revision Notes Chapter 2 Sexual Reproduction in Flowering Plants

Students of Class 12 Biology will be able to revise the entire chapter and also learn all important concepts based on the topic wise notes given below. Our best teachers for Grade 12 have prepared these to help you get better marks in upcoming examinations. These revision notes cover all important topics given in this chapter.

  • Sexual reproduction is the formation of new individuals through the meiotic gamete formation and their subsequent fusion during fertilisation. It is also called amphimixis. 
  • Flower is a specialised condensed shoot meant for carrying out the sexual reproduction. It bears floral leaves and gives rise to seeds and fruits.
  • A typical flower has 4 whorls of floral leaves viz., sepals (calyx), petals (corolla), stamens (androecium) and carpels (gynoecium). Stamens and carpels respectively represent male and female reproductive organs of flowers.


  • Androecium consists of stamens as its units. The stamens or microsporophylls are regarded as the male reproductive organs of the flower.
  • Each stamen consists of three parts – filament, anther and connective.
  • The lament is the slender stalk of the stamen, and the anther is the expanded head, borne by the filament. 
Sexual Reproduction in Flowering Plants Revision Notes
  • Each anther consists usually of two lobes connected together by a sort of midrib known as the connective.
  • Anther consists of 4 pollen sacs or microsporangia (two in each lobe). Microsporangial wall has four types of layers:
    (i) Epidermis : One cell thick and protective in function.
    (ii) Endothecium : Single layered, cells have cellulose thickening with a little pectin and lignin in some cases. It helps in anther dehiscence.
    (iii) Middle layers : 1-3 layers; they degenerate during maturity of the anther.
    (iv) Tapetum : Tapetal cells are nutritive. By these cells, the Ubisch bodies are produced which help in the ornamentation of microspore wall.
  • The process of formation of microspores from sporogenous tissue is known as microsporogenesis.
  • The sporogenous cells may directly function as microspore mother cells (also called pollen mother cells or PMCs).
  • As the anther matures and dehydrates, it dehisces by slits and pores to liberate the pollen grains.
  • Pollen grain (microspore) is the first cell of the male gametophyte.
  • Pollen grains are released from the anthers either at 2 – celled stage or at 3 – celled stage.
  • Wall or covering of pollen grain is called sporoderm. It has two layers, outer exine and inner intine. The outer layer exine is thick and sculptured or smooth. It is made up of sporopollenin which is resistant to chemical and biological decomposition so pollen wall is preserved for long periods in fossil deposits. At certain places the exine remains thin. These thin areas are known as germ pores, when they are circular in outline and germinal furrows when they are elongated. The intine is thin and elastic. It emerges out as the pollen tube from the germ pores during germination.
  • Development of male gametophyte is precocious, that is, it begins inside the microsporangium or pollen sac.
  • The size of the nucleus increases and protoplast then divides mitotically to produce a bigger vegetative or tube cell and a smaller generative cell.
  • The tube cell has a vacuolate cytoplasm which is rich in food reserve cell organelles and large and irregular nucleus.
  • The generative cell is small, spindle shaped to spherical shaped, with thin dense cytoplasm and a prominent nucleus.
  • Male gametes are formed by a mitotic division in a generative cell.


  • Gynoecium is female reproductive whorl of the flower. It is composed of one or more carpels or pistils.
  • Each pistil consists of three parts, i.e., stigma, style and ovary.
  • A pistil has a terminal receptive disc-like stigma, a stalk-like style and a basal swollen ovule bearing part called ovary. Inside the ovary is the ovarian cavity (with one or more locules) and  arenchymatous cushion called placenta, from which arise ovules.
  • An ovule or integumented megasporangium develops from the base or the inner surface of the ovary. It is small, generally an oval structure and consists chiey of a central body of tissue, the nucellus and one or two integuments. 
  • Each ovule is attached to the placenta by a small stalk called the funiculus.
  • The place of attachment of the funiculus with the main body of the ovule is called the hilum. 
Sexual Reproduction in Flowering Plants Revision Notes
  • In a typical (anatropous) ovule, the funiculus is fused with body of the ovule lengthwise beyond the hilum. It gives rise to a longitudinal ridge called raphe. Funiculus contains a vascular strand for the supply of nourishment to the  ovule.
  • The ovule contains a mass of thin walled parenchymatous cells called nucellus. The nucellus is protected by one or two multicellular coats called integuments. The basal portion of the nucellus from where the integuments appear is called chalaza. A small opening is le at the apex of integuments known as micropyle.
  • Embryo sac or female gametophyte is embedded in the micropylar half of the nucellus. This makes the most important part of the mature ovule. It is the embryo sac, which bears the embryo later on.
  • Formation of megaspore (n) from megaspore mother cell (2n) by meiosis inside the ovule is called megasporogenesis.
  • Only one magaspore mother cell remains functional and other 3 degenerate. This functional megaspore develops into a female gametophyte. This method of embryo sac formation from a single megaspore is called monosporic development.
  • The functional megaspore is the rst cell of female gametophyte. The nucleus of magaspore divides mitotically to form 8 nuclei and give rise to embryo sac or female gametophyte. This process is called megagametogenesis.
  • Out of 8 nuclei, 4 nuclei are arranged at each pole. One nucleus from each pole migrates to the centre to form two polar nuclei which further fuse to form a diploid or secondary nucleus.
  • Three nuclei at the base of embryo sac form antipodal cells. The remaining 3 nuclei at the micropylar end get surrounded by cytoplasm and are called egg apparatus. The egg apparatus consists of one larger middle cell known as egg or oosphere and the remaining two cells are called as synergids or help cells.
  • Thus a typical angiosperm embryo sac, at maturity, though 8-nucleate is 7 celled with 3 micropylar, 3 chalazal and one central cell.

Pollination refers to transfer of pollen grains from anther to stigma of a ower. It is of two type: self pollination and
cross pollination. 

Sexual Reproduction in Flowering Plants Revision Notes

Agents of pollination
The transfer of pollen grains from plant to plant in cross pollination takes place through biotic agencies (living agencies) or through abiotic agencies (non-living agencies).

Sexual Reproduction in Flowering Plants Revision Notes

Pollen – Pistil Interaction
Pollen – pistil interaction is a safety measure to ensure that illegitimate crossing do not occur. It checks the promotion or inhibition of pollen growth. Pollen grains of a number of plants may settle over a stigma but only the right pollen belonging to same species would germinate while others fail to do so. Compatibility and incompatibility of the pollen-pistil is determined by special proteins. The compatible pollens are able to absorb water and nutrients from the surface of the stigma. They germinate and produce pollen tubes. Pollen tubes grow into the style. Their growth and path through the style are also determined by specific chemicals.

Signifcance of pollination

  • Pollination  is a means of taking the male gametophyte for its growth near the female gametophyte.
  • Pollen-pistil interaction determines the suitability of pollen for carrying out the process of sexual reproduction.
  • It has freed the seed plants from the dependence on external water during fertilisation.
  • It can be manipulated to produce pure lines as well as desired varieties.


  • The fusion of male and female gamete is called fertilisation.
  • The fertilisation involving formation of pollen tube as in seed plants is called siphonogamy. On reaching the ovary, the pollen tube grows towards one of the ovules.
  • The pollen tube enters the ovule either through its micropyle (porogamy e.g., lily) or chalaza
  • (chalazogamy e.g., Betula) or integuments (mesogamy e.g., Cucurbita). Irrespective of the place of entry into ovules, the pollen tube enters the embryo sac through the micropylar region. 
  • Inside the embryo sac, one male gamete fuses with the egg to form the zygote (2n), the process is known as syngamy or generative fertilisation. The second male gamete fuses with 2 polar nuclei or secondary nucleus to form triploid primary endosperm nucleus, the process is known as triple fusion or vegetative fertilisation.
  • The zygote develops into embryo and primary endosperm nucleus develops into endosperm.
  • The occurrence of syngamy and triple fusion simultaneously in angiosperms is called double fertilisation. Double fertilisation was rst reported by S.G. Nawaschin (1898) in Fritillaria and Lilium.

The events of endosperm and embryo development, maturation of ovule(s) into seeds and ovary into fruit, are collectively termed as post-fertilisation events. 


  • As a consequence of triple fusion in which a male gamete fuses with the secondary nucleus, a triploid structure called primary endosperm nucleus (PEN) is formed that divides by mitotic divisions and forms a mass of nutritive cells called the endosperm.
  • Endosperm development precedes embryo development. Endosperm accumulates food reserves and functions as the nutritive tissue for the developing embryo. 
Sexual Reproduction in Flowering Plants Revision Notes


  • After fertilisation, the fertilised egg is called zygote or oospore which develops into an embryo.
  • This process of development of embryo is called embryogenesis.
  • The oospore before entering the process of embryogenesis undergoes a period of rest which may vary from few hours to few months.
  • Embryo develops at the micropylar end of the embryo sac where the zygote is situated. Most zygotes divide only aer certain amount of endosperm is formed.
  • A typical dicotyledonous embryo consists of an embryonal axis and two cotyledons. The part of  embryonal axis above the level of cotyledons is  called epicotyl. It terminates with the stem tip, called plumule (future shoot). The part below the level of cotyledons is called hypocotyl which terminates in the root tip called radicle (future root). The root tip is covered with a root cap (calyptra). In monocotyledons, there is only one terminal cotyledon situated towards lateral side of the embryonal axis called scutellum (shield shaped) e.g., grass family. At its lower end, the embxyonal axis has radicle and root cap enclosed in an undifferentiated sheath called coleorhiza. The part of axis above scutellum attachment is called epicotyl. It has shoot apex and few leaf primordia enclosed in hollow foliar structure called coleoptile. The early development of dicot and monocot embryos is similar upto globular stage.


  • Ripened ovules are known as seeds. A true seed is dened as a fertilised mature ovule that possesses an embryonic plant, stored food (sometimes absent) and a protective coat or coats.
  • Integuments of ovule harden as tough protective seed coat. Outer integument forms testa and tegmen develops from the inner integument.
  • Funiculus (stalk of ovule) forms stalk of the seed. Ultimately, stalk withers and leaves a minute scar called hilum. Antipodals, synergids disorganise and nucellus may disappear or persist as perisperm.
Sexual Reproduction in Flowering Plants Revision Notes
Sexual Reproduction in Flowering Plants Revision Notes


  • A fruit is a seed containing part of a plant that develops from a fertilised ovary and often from other tissues that surround it.
  • A pseudocarp or accessory fruit or false fruit is a fruit whose esh is derived from the ovary, along with some other floral part like thalamus, e.g., apple, strawberries.
  • A eucarp or true fruit is a fruit which is developed only from the ovary aer fertilisation, e.g., mango.
  • The wall covering the fruit is called pericarp. It consists of three parts :
    (i) Epicarp, called the skin of fruits.
    (ii) Mesocarp, middle eshy and pulpy part.
    (iii) Endocarp, innermost portion that surrounds the seeds.
  • Fruits can be simple fruit (develops from monocarpellary ovary), aggregate fruit (develops from multicarpellary apocarpous ovary) and composite fruits (develops from a complete inorescence).


Sexual Reproduction in Flowering Plants Revision Notes
Sexual Reproduction in Flowering Plants Revision Notes